BamH I

英文名称：BamH I

其他名称：限制性内切酶BamHI

CAS号：81295-09-2
级别：BR
来源：E.coli strain, that carries the cloned gene BamHI from Bacillus amyloliquefaciens H

活力定义：One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.

Recognition sites：G↑GATCC

Assayed on：Lambda DNA

缓冲液Optimal buffer：110 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT. +BSA

Optimal temperature：37℃

Storage conditions：10 mM Tris-HCl (pH 7.6); 50 mM NaCl; 0.1 mM EDTA; 100 μg/ml BSA; 1 mM DTT; 50% glycerol.

Ligations：After 50-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.

Non-specific hydrolisis：No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 37°C.

Methlation effect：Not blocked by Dam methylation (GmATC) GGATCC.

Thermal inactivation：enzyme is inactivated by incubation at 65°C for 20 mins.

兼容的末端：BclI, BglII, Bsp143I, MboI, PsuI.

使用说明：

1.High enzyme concentration may result in star activity.

2.Do not use BSA for long incubation.

3.To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 μg/ml.

性状(以下信息仅供参考)：液体
用途：本品仅供科研，不得用于其它用途。
保存：-20℃