英文名称:M63 Broth
级别:BR
成分(g/L)
硫酸铵:2.0
磷酸二氢钾:13.6
七水硫酸亚铁:0.5mg
PH@25℃:6.8~7.2
Additional supplements to be added affter autoclaving:
10ml of sterile 20% solution of glycerol
1ml of sterile 1M MgSO4 solution
The above solutions should be sterilized separately by filtration before addition to the broth.
Method of preparation:
1. Suspend 15.6g of M63 Medium broth in 1L of purified water
2. Allow the powder to dissolve.Heat and stir gently if required.Adjust pH to 7.0 with KOH
3. Autoclave at 121℃ for 15 minutes
4. Allow the solution to cool to 50℃ to bellow before adding antibiotics or additional nutritional supplements
性状(以下信息仅供参考):干燥粉末,易吸潮。参考用量:15.6g/L
用途:本品仅供科研,不得用于其它用途。(以下用途仅供参考)
保存:RT